干细胞研究院

About Institute

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姓名:刘海云

学位:博士

科研方向:

1.生化分析与生物传感

利用生物材料开展活细胞内肿瘤标志物的荧光检测和实时成像分析。

2.纸芯片研究与应用

设计开发实时、快速、个性化微流控纸芯片,开展疾病早期诊断研究。

主持项目:

1.国家自然科学基金青年科学基金项目:基于寡核酸探针信号放大microRNA活细胞内实时、动态荧光成像研究(21505052)(2016.01—2018.12)

2.中国博士后科学基金第61批面上资助:单细胞单分子microRNA定量成像及调控研究(2017M2169)(2017.03—2017.12)

3.山东省重点研发计划(公益性科技攻关类):用于长散在核元件快速检测的纸基生物芯片及其试剂盒的制备(2018GSF18023)(2018.01—2019.12)

4.山东省优秀中青年科学家科研奖励基金:用于肿瘤早期诊断的microRNA荧光检测与可视化成像(BS2014SW035)(2014.12—2016.12)

5.山东省高等学校科技计划项目:用于乳腺癌长散在核元件快速检测的研究(KJ2018BAC002)(2018.01—2020.12)

6.济南大学博士后科研启动基金:活细胞内癌症早期生物分子高灵敏分析检测研究(XBH1509)(2015.09—2017.09)

7.济南大学博士基金:癌症相关microRNA的荧光检测和可视化成像研究(XBS1458)(2014.10—2016.10)

代表论文:

1.Longhua Ding*, Lina Zhang, Hongmei Yang,Haiyun Liu*, Shenguang Ge, JinghuaYu. Electrochemical biosensor for p53 gene based on HRP-mimicking DNAzyme-catalyzed deposition of polyaniline coupled with hybridization chain reaction.Sensors and Actuators B: Chemical, 2018, 268, 210-216.

2.Tian Tian, Li Li, Yan Zhang,Haiyun Liu*, Lina Zhang, MeiYan*, JinghuaYu. Dual-mode fluorescence biosensor platform based on T-shaped duplex structure for detection of microRNA and folate receptor.Sensors and Actuators B: Chemical, 2018, 261, 44-50.

3.Yannan Jian, He Wang, Feifei Lan, Linlin Liang, Na Ren,Haiyun Liu*, Shenguang Ge*, Jinghua Yu. Electrochemiluminescence based detection of microRNA by applying an amplification strategy and Hg(II)-triggered disassembly of a metal organic frameworks functionalized with ruthenium(II)tris(bipyridine).Microchimica Acta, 2018,185(2),133-140.

4.Xiaolu Sun, He Wang, Yannan Jian, Feifei Lan, Lina Zhang,HaiyunLiu*, Shenguang Ge*, JinghuaYu. Ultrasensitive microfluidic paper-based electrochemical/visual biosensor based on spherical-like cerium dioxide catalyst for miR-21 detection.Biosensors and Bioelectronics, 2018, 105, 218-225.

5.He Wang, Yannan Jian, Qingkun Kong,Haiyun Liu, Feifei Lan, Linlin Liang, Shenguang Ge, Jinghua Yu. Ultrasensitive electrochemical paper-based biosensor for microRNA via strand displacement reaction and metal-organic frameworks.Sensors and Actuators B: Chemical, 2018, 257, 561-569.

6.Haiyun Liu, Lina Zhang, Mei Yan and Jinghua Yu*. Carbon Nanostructures in Biology and Medicine.Journal of Materials Chemistry B,2017, 5(32), 6437-6450.

7.Haiyun Liu, Chao Ma and Jinghua Yu*. A Simple and Apid Detection for Peptides Based on the Specific Recognition of Aptamer and Signal Amplification of Hybridization Chain Reaction.Nanomedicine: Nanotechnology, Biology and Medicine,2017.

8.Haiyun Liu, Tian Tian, Yuhan Zhang, Longhua Ding, Jinghua Yu and Mei Yan*. Sensitive and Rapid Detection of MicroRNAs Using Hairpin Probes-mediated Exponential Isothermal Amplification.Biosensors and Bioelectronics, 2017, 89, 710-714.

9.Hao Li, Fang Wang, Shenguang Ge,Haiyun Liu, Mei Yan*and Jinghua Yu. Turning Nonspecific Interference into Signal Amplification: Covalent Biosensing Nanoassembly Enabled by Metal-catalyzed Cross-coupling.Analytical Chemistry, 2017, 89(12), 6834-6839.

10.Tian Tian#,Haiyun Liu#, Li Li, Jinghua Yu, Shenguang Ge, Xianrang Songand Mei Yan*. Paper-based Biosensor for Noninvasive Detection of Epidermal Growth Factor Receptor Mutations in Non-small Cell Lung Cancer Patients.Sensors and Actuators B: Chemical, 2017, 251, 440-445.

11.Shenguang Ge, Feifei Lan, Linlin Liang, Na Ren, Li Li,Haiyun Liu, Mei Yan andJinghua Yu*. Ultrasensitive Photoelectrochemical Biosensing of Cell Surface N-glycan Expression Based on the Enhancement of Nanogold-assembled Mesoporous Silica Amplified by Graphene Quantum Dots and Hybridization Chain Reaction.ACS Applied Materials & Interfaces, 2017, 9, 6670-6678.

12.Chao Ma,Haiyun Liu, Lina Zhang, Hao Li*, Mei Yan,Xianrang SongandJinghua Yu. Multiplexed Aptasensor for Simultaneous Detection of Carcinoembryonic Antigen and Mucin-1 Based on Metal Ion Electrochemical Labels and Ru(NH3)63+Electronic Wires.Biosensors and Bioelectronics, 2018, 99, 8-13.

13.Longhua Ding,Haiyun Liu, Lina Zhang, Li Li and Jinghua Yu*. Label-free Detection of MicroRNA Based on the Fluorescence Quenching of Silicon Nanoparticles Induced by Catalyzed Hairpin Assembly Coupled with Hybridization Chain Reaction.Sensors and Actuators B: Chemical, 2017, 254, 370-376.

14.Chao Ma,Haiyun Liu, Lina Zhang, Li Li, Mei Yan, Jinghua Yu*and Xianrang Song*. Microfluidic Paper-based Analytical Device for Sensitive Detection of Peptides Based on Specific Recognition of Aptamer and Amplification Strategy of Hybridization Chain Reaction.ChemElectroChem, 2017, 4, 1744-1749.

15.Ren Na, Jianhua Li, Jichuan Qiu, Mei Yan,Haiyun Liu, Dandan Ji, Jiadong Huang, Jinghua Yu*and Hong Liu*. Growth and Accelerated Differentiation of Mesenchymal Stem Cells on Graphene-oxide-coated Titanate with Dexamethasone on Surface of Titanium Implants.Dental Materials, 2017, 33, 525-535.

16.Lu Li, Jie Feng,Haiyun Liu, Qingling Li, Lili Tong and Bo Tang*. Two-color Imaging of MicroRNA with Enzyme-free Signal Amplification via Hybridization Chain Reaction in Living Cells.Chemical Science, 2016, 7, 1940-1945.

17.Li Li, Yi Zhang, Lina Zhang, Shenguang Ge,Haiyun Liu, Na Ren, Mei Yan and Jinghua Yu*. Paper-based Device for Colorimetric and Photoelectrochemical Quantification of the Flux of H2O2Releasing from MCF-7 Cancer Cells.Analytical Chemistry, 2016, 88, 5369-5377.

18.Haiyun Liu,Tian Tian, Dandan Ji, Na Ren, Shenguang Ge, Mei Yan and Jinghua Yu*. A Graphene-enhanced Imaging of MicroRNA with Enzyme-free Signal Amplification of Catalyzed Hairpin Assembly in Living Cells.Biosensors and Bioelectronics. 2016, 85, 909-914.

19.Chao Ma,Haiyun Liu, Tian Tian,Xianrang Song, Jinghua Yu and Mei Yan*. A Simple and Rapid Detection Assay for Peptides Based on the Specific Recognition of Aptamer and Signal Amplification of Hybridization Chain Reaction.Biosensors and Bioelectronics, 2016, 83, 15-18.

20.Chunlei Yang, Xiu Wang, Lei Shen, Wenping Deng,Haiyun Liu, Shenguang Ge, Mei Yan*andXianrang Song. An Aldehyde Group-based P-acid Probe for Selective Fluorescence Turn-on Sensing of Cysteine and Homocysteine.Biosensors and Bioelectronics, 2016, 80, 17-23.

21.Linlin Liang, Feifei Lan, Li Li, Shenguang Ge*, Jinghua Yu, Na Ren,Haiyun Liuand Mei Yan. Paper Analytical Devices for Dynamic Evaluation of Cell Surface N-glycan Expression via a Bimodal Biosensor based on Multibranched Hybridization Chain Reaction Amplification.Biosensors and Bioelectronics, 2016, 86, 756-763.

22.Linlin Liang, Feifei Lan, Li Li, Mei Su, Shenguang Ge*, Jinghua Yu,Haiyun Liuand Mei Yan. Fluorescence "Turn-on" Determination of H2O2Using Multilayer Porous SiO2/NGQDs and PdAu Mimetics Enzymatic/Oxidative Cleavage of Single-stranded DNA.Biosensors and Bioelectronics, 2016, 82, 204-211.

23.Yan Zhang, Meng Li,Haiyun Liu, Shenguang Ge and Jinghua Yu*. Label-free Colorimetric Logic Gates Based on Free Gold Nanoparticles and the Coordination Strategy between Cytosine and Silver Ions.New Journal of Chemistry, 2016, 40, 5516-5522.

24.Haiyun Liu, Lina Zhang, Meng Li, Mei Yan, Mei Xue, Yan Zhang, Min Su, Jinghua Yu and Shenguang Ge*. Electrochemiluminescent Molecular Logic Gates Based on MCNTs for the Multiplexed Analysis of Mercury (II) and Silver (I) Ions.RSC Advance,2016, 6(31), 26147-26154.

25.Min Su,Haiyun Liu, Shenguang Ge, Na Ren, Longhua Ding, Jinghua Yu*and Xianrang Song. AnElectrochemiluminescence Lab-on-paper Device for Sensitive Detection of Two Antigens at the MCF-7 Cell Surface Based on Porous Bimetallic AuPd Nanoparticles.RSC Advance,2016,6, 16500-16506.

26.Xiu Wang, Lei Shen, Wenping Deng, Mei Yan*,Haiyun Liu, Shenguang Ge, Jinghua Yu and Xianrang Song. A Sensitive Electrochemiluminescent Immunosensor Based on 3D-flower-like MoS2Microspheres and Using AuPt Nanoparticles for Signal Amplification.RSC Advance,2016, 6, 23411-23419.

27.Ludan Wu, Yan Zhang, Yanhu Wang, Shenguang Ge,Haiyun Liu, Mei Yan and Jinghua Yu*. A Paper-based Electrochemiluminescence Electrode as An Aptamer-based Cytosensor Using PtNi@Carbon Dots as Nanolabels forDetection of Cancer Cells and for In-situ Screening of Anticancer Drugs.Microchimica Acta, 2016, 183, 1873-1880.

28.Yanhu Wang, Shenguang Ge,Haiyun Liu, Jinghua Yu*and Mei Yan. Paper-based Colorimetric Analytical Device Based on Molecularly Imprinted Polymers.Nanomedicine: Nanotechnology, Biology and Medicine, 2016, 12, 534-534.

29.Haiyun Liu, Chao Ma, Shenguang Ge, Mei Yan and Jinghua Yu*. Imaging of MicroRNA with Enzyme-free SignalAmplification of Catalyzed Hairpin Assembly in LivingCells.Nanomedicine: Nanotechnology, Biology and Medicine, 2016, 12(2), 512-512.

30.ShenguangGe, WeiyanLiu,HaiyunLiu,FangLiu, JinghuaYu, MeiYan*and JiadongHuang*. Colorimetric Detection of the Flux of Hydrogen Peroxide Released from Living Cells Based on the High Peroxidase-like Catalytic Performance of Porous PtPd Nanorods.Biosensors and Bioelectronics,2015, 71, 456-462.

31.Ludan Wu, Chao Ma, Xiaoxiao Zheng,Haiyun Liuand Jinghua Yu*. Paper-based Electrochemiluminescence Origami Device for Protein Detection Using Assembled Cascade DNA-Carbon Dots Nanotags Based on Rolling Circle Amplification.Biosensors and Bioelectronics,2015, 68, 413-420.

32.Yanhu Wang,Haiyun Liu, Panpan Wang, Jinghua Yu, Shenguang Ge and Mei Yan*. Chemiluminescence Excited Photoelectrochemical Competitive Immunosensing Lab-on-paper Device Using an Integrated Paper Supercapacitor for Signal Amplication.Sensors and Actuators B: Chemical,2015, 208, 546-553.

33.Chunlei Yang, Wenping Deng,Haiyun Liu, Shenguang Ge and Mei Yan*. Turn-onFluorescence Sensor for Glutathione in Aqueous Solutions Using Carbon Dots–MnO2Nanocomposites.Sensors and Actuators B: Chemical, 2015, 216, 286-292.

34.Shenguang Ge, Yan Zhang, Lina Zhang, Linlin Liang,Haiyun Liu, Mei Yan, Jiadong Huang and Jinghua Yu*. UltrasensitiveElectrochemical Cancer Cells Sensor Based on Trimetallic Dendritic Au@PtPd Nanoparticles for Signal Amplification on Lab-on-paper Device.Sensors and Actuators B: Chemical,2015, 220, 665-672.

35.Guoqiang Sun,Haiyun Liu, Yan Zhang, Jinghua Yu, Mei Yan*, Xianrang Song and Wenxing He*. Gold Nanorods-paper Electrode Based Enzyme-free Electrochemical Immunoassay of Prostate Specific Antigen Using Porous Zinc Oxide Spheres-silver Nanoparticles Nanocomposites as Labels.New Journal of Chemistry,2015, 39, 6062-6067.

36.Shenguang Ge, Lina Zhang, Yan Zhang,Haiyun Liu, Jiadong Huang, Mei Yan and Jinghua Yu*. Electrochemical K-562 Cells Sensor Based on Origami Paper Device for Point-of-care Testing.Talanta, 2015, 145, 12-19.

37.Haiyun Liu#, Lu Li#, Qian Wang, Lili Duan and Bo Tang*.Graphene Fluorescence Switch-Based Cooperative Amplification: A Sensitive and Accurate Method to Detection MicroRNA.Analytical Chemistry,2014,86(11),5487-5493.

38.Haiyun Liu#, Lu Li#, Lili Duan, Xu Wang, Yanxia Xie, Lili Tong, Qian Wang and Bo Tang*. High Specific and Ultrasensitive Isothermal Detection of MicroRNA by Padlock Probe-Based Exponential Rolling Circle Amplification.Analytical Chemistry,2013, 85(16), 7941-7947.

39.刘海云*,毛瑞之,田甜.活细胞MicroRNA成像研究进展.济南大学学报(自然科学版),2017,31(01),1-10.

40.程芳艳,王继亮,刘海云,王敏,杜维俊*.野生和地方大豆品种耐旱性初步评价.山西农业科学,2007,35(7),31-35.

41.刘海云*,姬丹丹.山西沁水食、药用植物资源调查.山西农业科学,2007,35(4),19-23.

42.刘海云,王敏,王继亮,程芳艳,杜维俊*.大豆SSR技术反应体系的优化.华北农学报,2007,22(5),36-39.

授权发明专利:

1.刘海云、祝琳、岳森、何涛、于京华,一种无酶标记DNA逻辑体系检测miRNA的方法,2017.09.30,201710910583.5

2.刘海云、谢青青、朱黎萍、田甜、常晓涵、蔡雷声、祝琳、颜梅、于京华,一种酶性DNA机器用于miRNA检测的方法,2017.07.17,201710581787.9

3.刘海云、常晓涵、赵帆、张玉诚、马超、谢青青、颜梅、于京华,一种灵敏定量检测ATP的方法,2017.07.04,201710535778.6

4.刘海云、蔡雷声、赵新玉、祝琳、张雪、颜梅、于京华,一种简单灵敏的超氧自由基和过氧化氢纸基检测方法,2017.06.30,201710519485.9

5.刘海云、杨浩、何涛、蔡雷声、颜梅、于京华,一种纸基灵敏检测miRNA的方法,2017.05.09,201710319907.8

6.刘海云、田甜、颜梅、于京华、葛慎光、张彦、马超,一种基于等温指数扩增法快速检测miRNA的方法,2016.07.14,201610551469.3

7.马超、于京华、刘海云、葛慎光、颜梅、孔庆坤、张彦、田甜,一种同时检测两种肿瘤标志物的电化学方法,2016.08.31,201610769134.9

8.王秀、颜梅、杨春蕾、张彦、于京华、葛慎光、刘海云,一种基于MoS2-PA-FA检测癌细胞上FR的方法,20160830,201610758494.9

9.葛慎光、兰飞飞、王贺、鉴燕楠、张彦、于京华、刘海云、颜梅,一种化学发光光致电化学传感器的制备及有机磷类农药检测,20160818,201610684000.7

10.葛慎光、兰飞飞、梁琳琳、鉴燕楠、王贺、王衍虎、于京华、颜梅、刘海云,一种金属有机框架材料表面负载分子印迹聚合物膜用于乙酰甲胺磷的电化学检测方法,20160818,201610684006.4

11.杨红梅、张彦、李丽、于京华、葛慎光、颜梅、刘海云,一种高灵敏的无酶电化学免疫传感器的制备方法及应用,20160809,201610643673.8

12.李丽、张彦、黄煜真、葛慎光、颜梅、刘海云、于京华,三维纸基电化学比率计的制备方法,20160727,201610595195.8

13.梁琳琳、葛慎光、于京华、兰飞飞、李丽、刘海云、任娜,三维金属增强荧光/比色双模纸芯片的制备及ATP检测,20160719,201610566988.7

14.梁琳琳、葛慎光、于京华、李丽、兰飞飞、任娜、刘海云,比率型三维金属增强荧光Pb2+生物传感器的构建,20160719,201610567187.2

15.马超、于京华、刘海云、颜梅、葛慎光、李丽、王秀、杨春蕾、张彦,一种灵敏检测小肽MUC1的方法,2016.07.14,201610551557.3

16.王秀、颜梅、于京华、葛慎光、刘海云、杨春蕾、张彦,基于红色荧光碳纳米材料的细胞探针的合成与应用,20160714,201610551556.9

17.葛慎光、梁琳琳、于京华、李丽、兰飞飞、任娜、刘海云、张彦、颜梅,癌细胞及细胞表面多糖检测荧光/比色双模式纸芯片的制备,20160602,201610384836.5

18.李丽、张彦、葛慎光、刘海云、于京华、颜梅,一种三维纸基金属有机框架的制备方法,20160524,201610346615.9

19.李丽、张彦、梁琳琳、葛慎光、刘海云、于京华,比率型电化学发光纸芯片的制备及在铅离子检测中的应用,20160524,201610346621.4

20.王秀、颜梅、于京华、葛慎光、刘海云、杨春蕾、张彦,一种检测活细胞中氧气含量的比率荧光探针的合成方法,20160428,201610271342.6

21.于京华、马超、刘海云、颜梅、葛慎光、张彦、李丽、田甜,一种快速检测肿瘤相关小肽MUC1的方法,2016.03.29,201610184950.3

22.李丽、于京华、张彦、苏敏、梁琳琳、葛慎光、颜梅、刘海云,一种三维比色和光电化学纸基设备的制备及其在过氧化氢检测中的应用,20160119,201610031944.4

23.王秀、颜梅、张彦、于京华、葛慎光、刘海云、杨春蕾,基于苯炔荧光试剂检测硫脲含量的新方法,20160107,201610006811.1

24.颜梅、邓文平、于京华、葛慎光、刘海云、张彦、申蕾、王秀、杨春蕾、朱少军、杨光鑫,检测循环肿瘤细胞的电化学免疫传感器的制备及应用,20150925,201510619623.1

25.于京华、吴鲁丹、刘海云、王衍虎、张彦、葛慎光、颜梅,一种合金纳米粒子修饰的电致发光细胞传感纸芯片的制备,20150515,201510246434.4

26.于京华、李丽、颜梅、葛慎光、刘海云、张彦、苏敏、刘芳、梁琳琳,一种电化学发光纸芯片的制备及其在硫化氢检测中的应用,20150515,201510245913.4

27.张丽娜、张彦、葛慎光、于京华、颜梅、刘海云、黄加栋、王衍虎、孙国强、李丽,一种二氧化锰修饰的玻璃纤维的制备方法,20150515,CN104876455B

28.张彦、于京华、张丽娜、葛慎光、颜梅、刘海云、王衍虎、孙国强、吴开庆、李丽、吴鲁丹,一种中空通道纸基折叠电池,20150330,CN104733773B

29.于京华、李帅、张彦、王衍虎、刘芳、颜梅、葛慎光、刘海云、张丽娜,一种简易、高通量微流控化学发光纸芯片的制备及应用,20150125,CN104624259B

30.于京华、王衍虎、葛慎光、李帅、高超民、颜梅、刘海云、张丽娜、张彦,一种可视化自供能葡萄糖生物传感器的构建方法,20141203,201410722008.9

31.于京华、王衍虎、葛慎光、李帅、高超民、颜梅、刘海云、张丽娜、张彦,一种纸基自供能生物传感器的制备方法及应用,20141201,201410706730.3

32.于京华、张彦、李念强、张丽娜、葛慎光、颜梅、刘海云、黄加栋、孙国强、吴开庆、吴鲁丹、苏敏,一种纸基比色检测葡萄糖的方法, 20141117,CN104374769B

33.颜梅、王秀、于京华、申蕾、邓文平、葛慎光、张彦、刘海云、杨春蕾,检测肿瘤标志物电致化学发光免疫传感器的制备及应用,20140924,CN104297479B

34.葛慎光、于京华、颜梅、黄加栋、张彦、刘海云、王衍虎,一种电聚合分子印迹聚合物中空通道纸器件的制备及在农药残留即时检测中的应用,20140831,CN104181215B

35.于京华、吴开庆、张彦、马超、孙国强、吴鲁丹、李丽、刘海云、颜梅、葛慎光,一种应用于燃料电池催化的花状多孔碳材料复合物的制备,20140731,CN104151821B

获得奖项:

1.2017年,“山东省新旧动能转换重大工程”专题研究征文三等奖

2.2017年,中国分析测试协会科学技术奖(CSIA奖)二等奖,第四位

3.2017年,济南大学化学化工学院科创优秀团队,指导教师

4.2017年,济南大学第四届科技创新大赛一等奖,指导教师

5.2017年,济南大学大学生研究训练计划(SRT),指导教师

6.2017年,第十五届济南大学“挑战杯”大学生课外学术科技作品竞赛三等奖,指导教师

7.2017年,济南大学先进班集体应化1403班,班主任

8.2017年,济南大学第三届科技创新大赛一等奖,指导教师

9.2016年,第三届山东省大学生科技创新大赛优秀指导教师

10.2016年,第三届山东省大学生科技创新大赛金奖,指导教师

11.2016年,济南大学大学生研究训练计划(SRT),指导教师

12.2016年,济南大学优秀班主任

13.2015年,济南大学化学化工学院优秀学士学位论文,指导教师

14.2015年,济南大学先进班集体化1406班,班主任

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